Journal article
TLR9 and TLR7/8 activation induces formation of keratic precipitates and giant macrophages in the mouse cornea
HR Chinnery, CM Leong, W Chen, JV Forrester, PG McMenamin
Journal of Leukocyte Biology | FEDERATION AMER SOC EXP BIOL | Published : 2015
Abstract
Macrophage adherence to the inner corneal surface and formation of MGCs in the stroma are common signs of chronic inflammation following corneal infection. To determine whether macrophage adherence (known clinically as KPs) and giant cell formation were specific to innate immune activation via particular TLR ligands, macrophage activation was examined in a murine model of TLR-mediated corneal inflammation. The corneal epithelium was debrided and highly purified TLR ligands were topically applied once to the cornea of, TLR7-l- , Cx3cngfp/+, CD11coYFP, and IL-4 -l- mice. At 1 week post-treatment macrophage activation and phenotype was evaluated in the cornea. Treatment with TLR2, TLR3, TLR4, a..
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Awarded by National Health and Medical Research Council Project Grants Australia
Awarded by Chief Scientist Office
Funding Acknowledgements
This study was supported by the National Health and Medical Research Council Project Grants Australia (#1026301 and #1042612). H.R.C. was supported by a Monash University Faculty Grant Scheme Early Career Research grant. The authors acknowledge the staff at Monash Micro Imaging Facility at Monash University (Victoria, Australia) and the Advanced Microscopy Facility at The Florey for provision of instrumentation, training, and general support. Janet Choi and Jun Shi (University of Melbourne) assisted with animal experiments and ELISA studies.